In order to effectively handle the challenges of our time, it is crucial that entrepreneurship education promotes the development of competencies that enable entrepreneurs to cope with artificial intelligence. One effective approach to achieve this is through the use of serious games. This article presents an overview of different types of serious games and their potential benefits for learning about artificial intelligence. It then focuses on ‘Cards for Entrepreneurship’, an analogue serious game which aims to stimulate the creative and critical thinking of entrepreneurs in relation to artificial intelligence by letting them actively think about challenges and opportunities. As part of a workshop, participants, engaged in both a rapid prototyping and a game play phase, were developing and playing ‘Cards for Entrepreneurship’. This approach was evaluated with a pre- and post-questionnaire, which revealed that the participants experienced an increase in their familiarity with artificial intelligence. Also, during the workshop a high level of flow, serious fun, and productive dialogues were observed. Future research should address the question of the extent to which artificial intelligence literacy can be developed in this context.