The cosmeceutical industry relies on a diverse range of advanced analytical methods to ensure the quality, safety, and efficacy of its products. This chapter explores key techniques, including chromatographic methods such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and thin-layer chromatography (TLC), which are essential for separating, identifying, and quantifying bioactive compounds and contaminants in cosmetic formulations. Spectroscopic methods, including UV-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy, offer critical insights into structural characterization and ingredient interactions. Microscopic and imaging techniques, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and confocal microscopy, provide detailed visualization of particle morphology and nanoscale interactions within formulations. Thermal analysis methods, such as thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), evaluate ingredient stability and product shelf life, while microbiological techniques like enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) ensure microbial safety. These analytical methods address the unique challenges of natural cosmeceuticals, including ingredient variability, degradation susceptibility, and compliance with global regulatory frameworks. In addition to these applications, the discussed techniques align with several sustainable development goals (SDGs). By ensuring the safety and efficacy of products, they contribute to SDG 3 (good health and well-being), safeguarding consumer health. Innovations in analytical approaches drive progress in cosmeceutical formulation and production, supporting SDG 9 (industry, innovation, and infrastructure). Green analytical methods, which reduce hazardous waste and conserve resources, promote SDG 12 (responsible consumption and production), while the sustainable sourcing and analysis of natural ingredients aid in conserving biodiversity, aligning with SDG 15 (life on land). Emerging trends, such as green analytical chemistry, high-throughput screening, and nanotechnology, are further shaping the future of cosmeceutical analysis, enhancing efficiency, precision, and sustainability. This chapter provides a comprehensive overview of these methods, highlighting their application in natural cosmeceutical products and their critical role in advancing a safer, greener, and more sustainable cosmeceutical industry.