Due to the lack of effective methodologies and instrumentation, limited studies have been conducted to analyze the tympanic membrane’s (TM, also known as eardrum) response under high-pressure blast waves. This research focuses on the characterization of a methodology that integrates high-speed 3D-Digital Image Correlation (HS 3D-DIC) and Schlieren imaging with a customized shock tube used for excitation to quantify rapid responses of the testing subject exposed to shock waves and their sound-matter interactions. The ultimate objective of our group’s research is to investigate the response and potential damage of human TM when exposed to high-level acoustic pressures. The system characterization encompasses the quantification of the shock tube’s output levels and repeatability, uncertainties in the full field-of-view measurement procedure, spatial and temporal resolutions of the imaging techniques, and the overall system limitations. By optimizing these parameters, we can enhance the reliability of high-speed imaging, ensuring accurate quantification of TM deformations during blast exposure. The accuracy of data obtained from HS 3D-DIC is validated by comparing them with simultaneous measurement by Laser Doppler Vibrometry. This study establishes a robust platform for comprehensive analysis of TM damage and fracture mechanics. Results showcase a validated full field-of-view measurement methodology from a real cadaveric human TM, including evaluation of its non-linear mechanical behaviors through dynamic responses and its interaction with overpressure blast waves. The tools we developed here will advance our understanding of blast-induced ear injuries and provide support for the development of effective protective measures and medical interventions.