Scholarlink

Graves Orbitopathy (GO), a severe complication of autoimmune thyroid disease, poses significant diagnostic and therapeutic challenges. This study explores the utility of tear film proteomics as a non-invasive method for identifying biomarkers in orbital inflammatory diseases, including GO. Using a prospective cohort design, tear samples were collected longitudinally from 125 subjects across various groups: Graves’ disease (GD) with and without orbitopathy, Hashimoto’s thyroiditis, thyroid cancer, nonspecific orbital inflammation (NSOI), granulomatosis with polyangiitis (GPA), sarcoidosis, Sjogren’s syndrome, and IgG4-related orbitopathy. Proteomic analysis was conducted using bottom-up liquid chromatography-electrospray ionization tandem mass spectrometry (BULCMS) to quantify differential protein expression and analyze pathways involved in inflammation and fibrosis. Results revealed unique proteomic profiles in tears from GO patients compared to controls and other orbital inflammatory disorders, highlighting potential diagnostic biomarkers and insights into pathophysiological mechanisms. This study underscores the translational potential of tear proteomics in precision medicine for orbital diseases, offering new avenues for early diagnosis, monitoring disease progression, and tailoring therapeutic interventions.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Proteomic Analysis and Quantification of Human Tears in Graves Orbitopathy (GO): A Novel Approach

  • 章节

  • 发表日期：

    2025年

查看全文
  • Hadi Khazaei

摘要

Graves Orbitopathy (GO), a severe complication of autoimmune thyroid disease, poses significant diagnostic and therapeutic challenges. This study explores the utility of tear film proteomics as a non-invasive method for identifying biomarkers in orbital inflammatory diseases, including GO. Using a prospective cohort design, tear samples were collected longitudinally from 125 subjects across various groups: Graves’ disease (GD) with and without orbitopathy, Hashimoto’s thyroiditis, thyroid cancer, nonspecific orbital inflammation (NSOI), granulomatosis with polyangiitis (GPA), sarcoidosis, Sjogren’s syndrome, and IgG4-related orbitopathy. Proteomic analysis was conducted using bottom-up liquid chromatography-electrospray ionization tandem mass spectrometry (BULCMS) to quantify differential protein expression and analyze pathways involved in inflammation and fibrosis. Results revealed unique proteomic profiles in tears from GO patients compared to controls and other orbital inflammatory disorders, highlighting potential diagnostic biomarkers and insights into pathophysiological mechanisms. This study underscores the translational potential of tear proteomics in precision medicine for orbital diseases, offering new avenues for early diagnosis, monitoring disease progression, and tailoring therapeutic interventions.