This chapter introduces the main aspects of Python programming relevant to biomedical data analysis. It begins with the basic syntax and operations that form the core of Python programming, providing a foundation for writing efficient and effective code. Key topics include variables, data types, and data structures, which are used to organize, store, manipulate, and manage information in complex biomedical datasets. The chapter also cover control structures, including loops and conditionals, which enable programs to make decisions and automate repetitive tasks. Together, these concepts offer a starting point for further exploration of advanced Python programming in biomedical applications.