There has been a significant surge of interest in minimally invasive regenerative therapies designed to alleviate pain, promote healing, and shorten recovery periods associated with injury. Platelet-rich plasma (PRP) has emerged as a prominent and widely accepted treatment modality within the field of orthopedics, particularly as a result of the increasing body of research elucidating its optimization and effectiveness. Among the various orthobiologics employed in orthopedic practice, PRP stands out as the most frequently utilized, particularly for conditions affecting joints, cartilage, tendons, muscles, ligaments, and meniscal injuries. Platelet-rich plasma is defined as any plasma sample derived from centrifuged autologous blood that exhibits a platelet concentration exceeding the baseline level prior to centrifugation. The α-granules contained within platelets harbor cytokines and growth factors that facilitate tissue repair through mechanisms such as angiogenesis, neovascularization, enhanced blood supply, and nourishment to adjacent cells, as well as promoting cell proliferation, chemotaxis, and differentiation. By isolating and concentrating these advantageous cytokines and growth factors, the healing process can be significantly improved when they are applied to injury sites. Although PRP can be utilized across a range of orthopedic conditions, it is important to recognize that it is not a “one-size-fits-all” solution. Given the diversity of PRP systems and preparations, a personalized approach is essential, aligning the specific pathology with the appropriate preparation. Not all pathological lesions respond uniformly, nor do all preparations yield equivalent results across different conditions. This chapter seeks to explore the applications of PRP in orthopedic treatment comprehensively.