The analysis of radiochemical composition is critical in the development and production of radiopharmaceuticals, for example, to assess synthesis performance, perform quality control testing, or analyze radiometabolites. In addition to widely used techniques based on liquid chromatography (e.g., HPLC) and planar chromatography (e.g., TLC), high-performance methods based on electrophoresis are also used in a wide range of applications. This chapter reviews the techniques of gel electrophoresis, paper electrophoresis, capillary electrophoresis, and microchip electrophoresis, and selected applications in the field of radiopharmaceutical analysis.