Aristolochia acids (AAs) are found in the Aristolochiaceae family, which includes about 500 species, spread all around the world, with most of the taxa in the tropical region and are widely used as herbal remedies. In the 1990s, their use for weight loss in developed countries was prohibited or strictly limited. Since then, AAs have been produced commercially only as a reference standard and as research chemicals. The occurrence of AAs in either the above- or underground parts of vegetables is attributed to a specific passage route that could explain how AAs are transported within the environment. Some results show that AAs’ presence is attributed to the growing of A. clematitis in the area, and in general, the highest concentrations are found in the soil samples, and the lowest concentrations in cucumber and fruit, but without a direct correlation to the distance (or 50 cm) from the Aristolochia plants. Aristolochic acid adducts and DNA were found in urothelial tissue samples from all urothelial cancer patients with Chinese herbal nephropathy. Their urinary metabolites are aristolactam I (a metabolite of aristolochic acid I) and aristolactam II (a metabolite of aristolochic acid II). Apart from aristolactam, DNA adducts are also their urinary metabolites.