The introduction justifies the relevance of the stated topic and presents the author’s position regarding the specificity of forming students’ environmental culture in the learning process. This becomes possible only with the integration of environmental knowledge into the content of general and professional training courses in the aspect of the studied discipline. In the “Methodology” section, the authors defined the system of tasks for environmental education of student youth and described the role of the academic discipline “Physical Education and Sports” as an integral part of the learning process and the formation of the personality of a student (in perspective, a professional specialist). The direction of the research is clarified. The conduct of an exploratory experiment is described to study the presence or absence of information about ecology in the knowledge of first-year students and determine the level of their environmental culture. Survey results indicate that first-year students, having a generally fragmented and non-systemic understanding of ecology and environmental issues, show little interest in such knowledge, resulting in an average level of environmental culture. Further, the authors described methods and techniques of the work of physical education teachers with first-year students in lectures and practical classes. Following the study of the academic discipline “Physical Education and Sports” in the first year, a final assessment is provided in the form of a survey. The analysis of the results demonstrated an increased interest in environmental issues, a deeper understanding, and an elevated level of individual environmental culture for each student. The “Conclusion” section briefly summarizes the research findings.