Cell culture is a powerful tool for biological investigation and one of the most widely used approaches to mammalian biology. This technique allows rapid iteration with lower costs than other methods, particularly animal models. At the same time, it is sufficiently complex to model various intracellular processes reliably. An understanding of the technique is essential to a modern scientific career. This chapter introduces a basic framework for understanding key ideas in cell culture.

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Effective Cell and Tissue Culture

  • Neil B. Blok,
  • Cody M. Dalton,
  • Sean M. Ronnekleiv-Kelly,
  • Troy A. Markel,
  • Christina V. Angeles

摘要

Cell culture is a powerful tool for biological investigation and one of the most widely used approaches to mammalian biology. This technique allows rapid iteration with lower costs than other methods, particularly animal models. At the same time, it is sufficiently complex to model various intracellular processes reliably. An understanding of the technique is essential to a modern scientific career. This chapter introduces a basic framework for understanding key ideas in cell culture.