Sample Preparation for Soft Matters (Non Biological) and Biological Samples

This chapter dedicatedly addresses requirements related to sample preparation and analysis of specimen belong to soft matter and biological samples through TEM technique. Chapter begins with overview of stated samples classification w.r.t. TEM analysis. Most importantly, importance of fixation with detail of different type of fixative, their properties, dehydration, embedding medium, block preparation followed by ultrathin sectioning have discussed. During ultrathin sectioning and precise handling w.r.t. their transferring onto grid surface, staining and respective storage etc., also has been mentioned in detail w.r.t. precise TEM imaging. In addition, challenges w.r.t. soft and biological sample preparation and imaging has been discussed with optimization towards ideal imaging. Ultimately, current chapter will equip researchers/scientist with extraordinary skills, technical expertise during soft and biological sample consistent and authentic TEM imaging database.