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The comet assay is a versatile method used to determine the DNA damage in individual cells. The cells processed by this technique preserve the stable genetic material in the head of the comet and the unstable portion of DNA in the tail. The analysis of the resulting microscopic images from this test must be carried out by an expert, who must precisely determine the extent of DNA liberated from the head of the comet, since it is related to the level of damage. Since this is a time-consuming and very specialized task, the objective of this research is to develop a computational system, based on the use of a convolutional neural network, for the automatic classification of cells processed by comet assay according to the level of DNA damage they present.

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Convolutional Neural Networks for the Classification of Comet Assay Cells

  • 会议论文

  • 发表日期：

    2025年

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  • Ismel Rizo-Blanco,
  • Anabel Martin-Gonzalez,
  • Carlos Brito-Loeza,
  • Ricardo Legarda-Saenz,
  • Arturo Espinosa-Romero,
  • Laura Lafon-Hughes

摘要

The comet assay is a versatile method used to determine the DNA damage in individual cells. The cells processed by this technique preserve the stable genetic material in the head of the comet and the unstable portion of DNA in the tail. The analysis of the resulting microscopic images from this test must be carried out by an expert, who must precisely determine the extent of DNA liberated from the head of the comet, since it is related to the level of damage. Since this is a time-consuming and very specialized task, the objective of this research is to develop a computational system, based on the use of a convolutional neural network, for the automatic classification of cells processed by comet assay according to the level of DNA damage they present.