Promoting onboard bicycling is crucial for advancing seamless transportation. A comprehensive understanding of factors influencing onboard bicycle carriage is essential for strategically planning new initiatives and enhancing existing services. However, studies on service quality evaluation and service quality indicators concerning onboard bicycling are unavailable in the literature. A questionnaire-based primary survey was conducted to understand the user perception of factors affecting onboard bicycle carriage facility provision associated with the Kochi metro service in India. User perception-based importance and satisfaction ratings were collected on 20 attributes concerning onboard bicycling. Considered attributes encompass station facilities, metro services, fare, reliability, and safety. Importance Satisfaction analysis (ISA) is employed to determine the relative importance and satisfaction of factors affecting the service. ISA helps to categorize the attributes into four groups, identifying the focus areas for infrastructure and service improvements. The analysis results indicate that the station and metro service-related attributes are more significant than factors such as real-time information availability and availability of staff services. The attributes that require immediate attention are those perceived as very important by the users but reported as unsatisfied with the service. These factors include the maintenance of the specific provisions at the station, such as the fare gate, vertical movement etc., ease of movement through fare gates into the paid ticket area, and the availability of special provisions exclusive to vertical bicycle movement. Understanding the influence of the various attributes can be utilized to prioritize infrastructure or service improvement, facilitating existing users and attracting new ones.