This work studies the production of bio-oil from the pyrolysis of residual biomass from eucalyptus (RBE) using a prototype auger reactor at 550 ºC. After production, the bio-oil was separated into heavy (HF) and light (LF) fractions. The water content, pH, heating value and elemental composition (CHNO) of these fractions were analyzed. The HF constituted 22–34%wt. of the bio-oil and presented a darker color, higher viscosity and lower water content (22–30%wt.) in comparison to the LF, which constituted 66–78%wt. of the bio-oil. The higher heating value (HHV) of HF ranged from 21.22 to 23.73 MJ/kgHF, while LF varied significantly from 2.48 to 8.48 MJ/kgLF. Elemental analysis of HF from the pyrolysis of eucalyptus branches and leaves showed similar results, with carbon content between 0.51 and 0.52 kgC/kgHF, hydrogen of 0.09 kgH/kgHF, and nitrogen of 0.01 kgN/kgHF. Oxygen content, including that in water, ranged from 0.38 to 0.39 kgO/kgHF. HF shows potential as a precursor for fuels but requires further investigation to address issues related to its high oxygen content and relatively low calorific value compared to conventional fuels. The water content and HHV of HF fall within the limits set by ASTM D7544-23 for commercial or industrial burner applications adapted to liquid pyrolysis biofuels, warranting further research for its use.