The activity of waste collection is essential for society. However, developing this activity may cause risks of work-related musculoskeletal disorders (WMSDs). The aim of the present study is to investigate the prevalence of musculoskeletal disorders in door-to-door municipal solid waste collectors in three municipalities in northern Portugal. For this, a psychophysical questionnaire was developed to obtain information about the characteristics of the population and Nordic Musculoskeletal Questionnaire (NMQ) were used to identify effort and health-related complaints. In the present study more than 85.2% of the workers reported symptoms of pain and discomfort in the last 12 months. The lumbar region had the highest number of musculoskeletal complaints 65%, then the shoulders 63%, and the knees 50%. There was no statistical significance between the variables age, length of experience, smoking, drinking and regular physical activity and the occurrence of musculoskeletal disorders, as no p-value was less than 0.05 with a 95% confidence level.