Lipopeptides are amphiphilic compounds since their molecular structure is formed by a fatty acid, which gives them hydrophobic characteristics. They are covalently linked to a hydrophilic peptide sequence that has between 7 and 10 amino acid residues. Lipopeptides are bioactive agents synthesized by the nonribosomal pathway in different species of bacteria and fungi. These biosurfactant compounds present a high biodiversity since they can present variations in the size and saturation of the fatty acid chain and the sequence, composition, and size of the peptide fraction. The high biodiversity of lipopeptides induces a wide range of properties such as surfactants, immunomodulators, antitumors, bioremediators, and mainly antimicrobials. The antimicrobial potential of lipopeptides is related to their ability to bind to the lipid molecules of cell membranes, where, depending on the concentration, they can cause alterations in the structure and permeability of the membranes, which can trigger cell death. The structural and functional diversity of lipopeptides has applications in different sectors, such as the food industry, where they can be used as emulsifiers and antimicrobials; in the recovery of crude oil from contaminated marine environments; in the agricultural sector as biocontrol products to reduce or eliminate diseases caused by plant pathogenic microorganisms, among others. Genetic engineering, metabolic engineering, and synthetic biology tools are used to increase the biodiversity, performance and effectiveness of lipopeptides to expand and improve their application in industrial activity.