Design and Development of a Bone-on-a-Chip System to Generate Microphysiologic Models
摘要
We introduce a bone tissue bioreactor system that features a hydraulic camera, which recreates the osmotic exchange of nutrients in the circulation of bone tissue, along with microfluidic interactions. This custom-designed system incorporates a double inlet-outlet bioreactor, equipped with peristaltic flow and adjustable velocities variations to regulate the luminal flow rate. The model was designed in Solidworks and simulated in COSMOSFloworks with the Universal physical water properties included an isothermal, Newtonian liquid, 1000 kg/m3 density, 0.889 mPa viscosity, a continuous flow rate of 7 ml/h, and atmospheric pressure, the material was photocurable resin (Anycubic), with Elasticity modulus (E) 1.2 GPa and Poisson ratio 0.3. Furthermore, the system effectively facilitates nutrients distribution in the bone tissue model, transferring substances in the chamber through the channels. This microfluidic system closely mimics the human bone, the optimum design simulation presents a flow of 12.43 nl/s, with a minimum pressure drop of 131.32 mPa, addressing many limitations and accuracy issues associated with previously established in vitro bone models, thereby offering a more representative simulation of bone tissue functions.