Nowadays, there is growing interest in anaerobic digestion of energy crops, leftovers, and wastes to lower greenhouse gas emissions and promote a sustainable growth of the energy source through biogas. The objective of this experiment was to test the biogas production potential from a mixture of food waste through anaerobic batch syringe (reactor) test method. An initial 3-mL volume of feed substrates (C and D) was used. Substrate C is made by mixing yeast and cream, while substrate D was a mixture of apple juice, yeast, and cream. After 7 days measurement, refeeding of substrates to the sample was done. The experiment is performed in series of 100-mL medical syringes which are used as small anaerobic digesters, AD, at 35 °C for all syringes before refeeding. After refeeding some syringes were tested at ambient temperature to see the effect of temperature. A better volume of biogas was obtained when the refeeding value was higher, and the temperature was 35 °C (C2_30_1). The average biogas production at the end of the day for C2_30_1 was 310 mL. On the other hand, lower biogas production was recorded in D2_10_1 (only 90 mL). That is, D2_10_1 is less than C2_30_1 by 70.9%. The refeeding volume of sample C2_30_1 was 10 mL, and a 3-mL volume refeed was used for D2_10_1. Different factors can affect biogas production potential. S/I ratio, inoculum adaptation, and substrate composition were the major factors identified affecting the biogas production.