This chapter of Women Scientists in American Television Comedy: Beakers, Big Bangs and Broken Hearts discusses the analysis of 47 episodes from Never Have I Ever, The Big Bang Theory and Zoey’s Extraordinary Playlist focusing on how humor types such as irony and satire are used to portray women scientists. These humor types, aligning with the Superiority Theory, often involve outwitting others and ridiculing male characters, highlighting the women scientists’ intellectual and social superiority and reflecting feminist humor’s subversive nature. The study found that satire and irony, more appreciated by a general and adolescent audience, were prevalent, with farcical humor also commonly used to depict scientific work humorously. Humor is also used to address gender biases in workplace dynamics, with male colleagues often dismissing women scientists, thereby mirroring real-world gender biases in STEM fields. The portrayal of work-life balance struggles for women scientists frequently employs schadenfreude and ironic humor, emphasizing the stereotype that women must choose between a career in STEM and their personal life. Additionally, humor derived from traditional gender stereotypes challenges their femininity and reinforces the incongruity of motherhood and STEM careers. The concept of the comic frame is used to examine how these shows employ humor as a tool to distance the audience, allowing viewers to engage critically with gendered stereotypes of women in science. Despite some positive portrayals, the humor often perpetuates stereotypes, potentially influencing young girls’ perceptions of STEM careers negatively. However, feminist humor in these shows also serves to critique and subvert patriarchal norms. This chapter illustrates both the empowering and limiting potential of comedic representation in shaping societal attitudes toward women scientists.