This chapter of Women Scientists in American Television Comedy: Beakers, Big Bangs and Broken Hearts examines how humor in TV comedies like Never Have I Ever, The Big Bang Theory and Zoey’s Extraordinary Playlist communicates and challenges social conventions about science and gender. The analysis reveals that irony (18%) and satire (17%) are the most common humor types used to portray women scientists, often emphasizing their expertise and competence in contrast to their male counterparts. For example, The Big Bang Theory uses deadpan and farcical humor to highlight the scientific prowess of characters like Amy Farrah Fowler. Humor also addresses the dynamics between male colleagues and women scientists, often depicting women as superior in competence, though this is not acknowledged by their male peers, using irony, satire and schadenfreude. Additionally, the chapter explores how humor reinforces or challenges stereotypes of women scientists, such as the “old maid” and “gruff women’s libber”, which suggest that intelligence and femininity are mutually exclusive. Traditional gender stereotypes are also addressed through ironic humor, subverting notions of domesticity and motherhood. The analysis demonstrates that humor in these TV comedies can simultaneously critique and perpetuate gender stereotypes in STEM fields, highlighting the complexities and nuances of these portrayals.