A preliminary experiment of investigating the permeability behavior of the twill flax preform has been conducted by having a typical set up of the vacuum infusion process. The effect of the flax preform is studied with regards to the effect of various stacking sequences on permeability behaviors. The study has also focused on the influence of these preforms on the final thickness and the total filling time for the completion of the impregnation process. It can be concluded from the findings that the preform stacking sequences affect the permeability behaviors, final thickness of preform and the total filling time significantly. The experimental data also reveals that the longer filling time is taken by the preform samples with the unidirectional flax preform with 0° and 90° to the in-plane resin flow direction when compared with the [+ 45°/− 45°] and [+ 45°/− 45°/− 45°/+ 45°]. In the future, a study is recommended to be further conducted to identify the effect of other factors such as compaction pressure, variation of mesh and/or the use of core and compared with the simulation works.