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In this comprehensive exposition, we undertake a meticulous exploration of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system. This architectural marvel has undergone a metamorphic evolution through the skilled engineering hands, establishing itself as an avant-garde force in the intricate realm of genome editing within molecular biology. Distinguished by a singular stylistic approach, we cast an illuminating focus upon the manifold applications and persistent enigmas enveloping CRISPR/Cas, with a discerning emphasis on the CRISPR/SpCas9 variant. Our scholarly journey navigates the complex landscape of natural product discovery, traversing a taxonomic spectrum that includes resilient bacterial entities like Streptomyces, Corynebacteria, and Myxobacteria, as well as the enigmatic territories of filamentous fungi, such as Aspergillus, Beauveria, and Ganoderma. Expanding beyond, our discourse extends its purview to encompass microscopic wonders in the form of microalgae and the verdant realms of plants.

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CRISPR/Cas-Based Genome Alterations in Natural Product Discovery

  • 章节

  • 发表日期：

    2025年

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  • Rishabh Kathuria,
  • Vibha Rani

摘要

In this comprehensive exposition, we undertake a meticulous exploration of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system. This architectural marvel has undergone a metamorphic evolution through the skilled engineering hands, establishing itself as an avant-garde force in the intricate realm of genome editing within molecular biology. Distinguished by a singular stylistic approach, we cast an illuminating focus upon the manifold applications and persistent enigmas enveloping CRISPR/Cas, with a discerning emphasis on the CRISPR/SpCas9 variant. Our scholarly journey navigates the complex landscape of natural product discovery, traversing a taxonomic spectrum that includes resilient bacterial entities like Streptomyces, Corynebacteria, and Myxobacteria, as well as the enigmatic territories of filamentous fungi, such as Aspergillus, Beauveria, and Ganoderma. Expanding beyond, our discourse extends its purview to encompass microscopic wonders in the form of microalgae and the verdant realms of plants.