The YABBY transcription factor family is a group of plant-specific genes known for their role in regulating organ development and patterning in various plant species. The expansion of this gene family could play a crucial role in enhancing plants’ adaptability and evolutionary potential by contributing to diverse developmental processes. This study aims to investigate the YABBY gene family in cassava (Manihot esculenta) through a comprehensive analysis of gene duplication events, categorization of family members, and gene structure. As a result, a total of 4 duplication events were identified, with duplicated genes localized on different chromosomes, indicating segmental duplication. The Ka/Ks ratio analysis suggested that these duplicated genes are under positive selection. Phylogenetic analysis categorized the YABBY transcription factor family into 5 groups. Gene structure analysis revealed variability in exon numbers. Our findings provided valuable insights into the evolutionary dynamics and functional diversity of the YABBY gene family in cassava, laying the groundwork for future research on their roles in plant development and stress response.