Full thickness burns are a serious public health problem that could potentially lead to death especially if they are not well handled. Despite advancement of medical sciences, wound management is still lacking on the ideal conditions that could produce optimal and quick healing. In this study, polymeric wound scaffolds were fabricated using an electrospinning method through the formation of single, mix, and double layers of polyvinyl alcohol (PVA) loaded with epidermal growth factor (EGF) and fibroblast growth factor (FGF). The fabricated scaffolds were subjected to in-vivo study by evaluating the scaffold implantation on full thickness burn wound of rats. Sixty-three male Sprague-Dawley rats were grouped into seven as control, burnt, PVA/EGF (single layer), PVA/FGF (single layer), PVA/EGF/FGF (mix layer), and PVA/EGF-FGF (double layer) nanofibers. On days 14 and 21, the skin samples of treated rat models were collected and subjected to histopathology using Masson’s trichrome stain. The results of this in-vivo study indicated that the PVA/EGF-FGF and PVA/EGF/FGF groups cause the generation of greater matured collagens with few hair follicles compared to the other groups at day 21. Thus, the incorporation of EGF and FGF in the PVA nanofibers is adoptable as a successful biological burn wound dressing scaffold.