Aortic dissection (AD) is a vascular disease characterized by high mortality rate which occurs most frequently in patients aged between 60 and 70 years. The number of patients with AD is increasing in Japan, as the country is slowly approaching the super-aging society. Blood vessel prosthesis implantation or stent-graft insertion is the common surgical procedure to treat the lesions with AD. High blood pressure and blood flow are often considered to be related to the development of AD. The purpose of this study was to investigate the potential involvement of von Willebrand factor (VWF), a plasma glycoprotein with blood coagulation properties and a high molecular weight, in the AD pathophysiological process. An original in vivo dissection model was created in the aorta of four healthy goats to represent the effectiveness with the intervened surgical treatment. We collected blood samples under the pre-operative and the AD, and measured high molecular weight multimer distribution with Western blot method after the plasmaization of blood samples. As a result, samples collected under the AD increased large multimer index (LMI) of VWF compared to the pre-operative blood samples. The results of this study showed that the VWF change in the original in vivo models were significantly influenced by the deterioration of AD.