Wild edible plants (WEPs) have always been an essential nutraceutical resource, given their nourishing value in times of food shortage, uses in folk medicine, and ethnographic relevance. Even though their use has declined over time, there has been a growing interest in the recent years for the application of WEPs as they can be an asset to meet the needs for food facing the rapid population growth, global warming, and dietary diversification. Pennyroyal (Mentha pulegium) is an endemic aromatic herb that grows wildly in the Mediterranean basin. Aqueous and acetonic extracts of the aerial parts of pennyroyal (wild and commercial) were obtained. Bioactive properties were evaluated in terms of antioxidant capacity (ABTS assay), total phenolic content (Folin-Ciocalteau assay) and antimicrobial activity against Gram-positive (Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumanni). All extracts presented antioxidant capacity, especially the aqueous extracts obtained from wild specimens, due to the presence of phenolic compounds, as proved by the positive correlation (Pearson coefficient 0.89) between the ABTS/TPC assays. Pennyroyal showed antimicrobial activity against Gram-positive bacteria.