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First and foremost, of course, is crystallisation for the crystallographic study (see e.g. Chayen et al. in Macromolecular Crystallization and Crystal Perfection, 2010) and single particle grids for cryoelectron microscopy (cryoEM) work (see e.g. Weissenberger et al. in Nat Methods 18:463–471, 2021). Secondly, an evaluation of whether your crystal diffracts, and whether it cools in an acceptable manner for cryo work should be done. Likewise, for cryoEM it can be the case that the access to a‘high end microscope’ at a central facility requires demonstration of the quality of the grids.

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Guidance in Preparing the Experiment at Your Home Facility Including Sample Preparation

  • 章节

  • 发表日期：

    2025年

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  • John R. Helliwell

摘要

First and foremost, of course, is crystallisation for the crystallographic study (see e.g. Chayen et al. in Macromolecular Crystallization and Crystal Perfection, 2010) and single particle grids for cryoelectron microscopy (cryoEM) work (see e.g. Weissenberger et al. in Nat Methods 18:463–471, 2021). Secondly, an evaluation of whether your crystal diffracts, and whether it cools in an acceptable manner for cryo work should be done. Likewise, for cryoEM it can be the case that the access to a‘high end microscope’ at a central facility requires demonstration of the quality of the grids.