As particle preservation techniques at cryo-temperatures improved, as well as the sensitivity of detectors, and their time-resolution, to the electrons transmitted through the single particles improved, the spatial resolution achievable with electron microscopy took a revolutionary leap to the atomic level. This was about ten years ago. There is an interesting restriction that the macromolecules or their complexes need to be a certain size such as above 100 kDa, depending on the spatial resolution achieved.

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Electron Biological Imaging Centres at the SR Facilities: Diamond, ESRF, Soleil and SSRL/SLAC as Examples

  • John R. Helliwell

摘要

As particle preservation techniques at cryo-temperatures improved, as well as the sensitivity of detectors, and their time-resolution, to the electrons transmitted through the single particles improved, the spatial resolution achievable with electron microscopy took a revolutionary leap to the atomic level. This was about ten years ago. There is an interesting restriction that the macromolecules or their complexes need to be a certain size such as above 100 kDa, depending on the spatial resolution achieved.