The protein called silk fibroin, which comes from the Bombyx mori silkworm, has exceptional mechanical, biodegradable, and biocompatibility properties that make it a great biomaterial for use in medical applications. Chemical-intensive methods that endanger human health and the environment are used to create silk. Green synthesis, a novel and environmentally friendly method, had to be developed for such a reason. Applications for silk fibroin include wound healing, tissue engineering, bone engineering, and many more. The advantages of green synthesis over traditional methods for creating silk fibroin biomaterial scaffolds for biomedical applications are explored in this chapter. Many techniques and alternatives to conventional techniques are covered, including the use of DES, enzymatic degumming, supercritical CO2 fluid, and many more. In conclusion, the mentioned observations indicate that green synthesized silk fiber scaffolds, which are also more environmentally friendly, can be used to produce enhanced silk fiber (SF) for biomedical applications.