Prairie gentianPrairie gentian or lisianthus (GentianaceaeGentianaceae) is a beautiful cut flowerCut flower valorized by its long-time vase lifeVase life. This speciesSpecies native to the southern United States and northern Mexico shows a lot of variations in colorsColor, shapesShape, and sizesSize. Due to its ornamentalOrnamental characteristics, it has been widely disseminated and its demand has increased. Based on its growing demand and morphological characteristics could be considered as the new roseRose. Its domesticationDomestication and variabilityVariability generation have been performed since 1930 and resulted in numerous commercial cultivarsCultivars. Some problems appear in the commercial culture, when temperatures exceed 25 °C it increases the percentage of plants with rosette formation in almost all commercial lisianthus cultivarsCultivars and reduces or delays bloom. This process in lisianthus is controlled by external and internal factors which determine successful pollinationPollination and seedSeed productionProduction. Among these, temperature and light qualityQuality are key factors. On the other hand, disease prevention is another concern in lisianthus breedingBreeding. Insects such as thrips, caterpillars, and leafminers, and fungi such as Rhizoctonia sp, FusariumFusarium sp., and BotrytisBotrytis cinerea, are some of the pathogensPathogen that negatively affect prairie gentianPrairie gentian productionProduction. In this sense, biotechnological tools could be used for geneticGenetic manipulation to solve some of these problems. Transgenic plants could be pathogenPathogen resistant, earlier in bloom, and show other characteristics of interest to improve plant and flowerFlower productionProduction and reduce time and cost of productionProduction.