Aim: The main aim of the study was to develop a sustainable serviette using a novel Pseudosasa japonica fiber sample with superior absorption and compare it with the efficiency of a locally available sanitary napkin. Materials and Methods: Processed bamboo fibres, neem leaves, cotton fabric and citric acid binder and other essential chemicals were used. Brauner-Emmett-Teller isotherm was used to evaluate the porosity and surface adsorption efficiency. Fourier Transform Infrared Spectroscopy was used to analyse the presence of functional groups present in the novel bamboo fibers. The prepared napkin was evaluated for its absorbency, test fluid, rewet under load, retention % and G power was 80%, the coincidence interval of 95% including group 1 and group 2 of 2 samples each. Results: The present study reported the performance of sustainable and phthalate free napkins made up of novel bamboo fibres and other natural ingredients. The observed results were highly promising in terms of porosity, adsorption index (7.86), antimicrobial efficacy and biodegradability. SPSS statistical analysis results depicted that the value of p was 0.001 (p < 0.05) using sample T test and which confirmed the statistical significance. Conclusion: Thus, this study was helpful to develop a simple bio-friendly sanitary napkin which was free from toxic substances with exclusive adsorbing efficacy.