The widespread use of pesticides in industry and agriculture persists in environmental bodies posing significant risks to human health and the environment. Conventional bioremediation has limitations in terms of precision and efficiency but nano bioremediation is a unique approach that combines the properties of nanomaterials with the augment of pesticide bioremediation efforts. It is an innovative strategy integrating nanobiotechnology and bioremediation techniques called nano bioremediation which offers a viable approach to deal with the urgent problem of pesticide pollution in the environment with enhanced efficiency for treating these pollutants. The nanostructures act as carriers with immobilized or encapsulated remediating microbial enzymes which can facilitate the bioremediation of such hazardous materials with improved stability and delivery to the target sites. The elimination of harmful pesticide contaminants using the synergistic effect of nanoparticles and microbial technologies along with the application is the primary aim of this chapter. Also, the importance of unique nano bioremediation techniques as a sustainable and cleaner technology for pollutant remediation will be discussed.