Water is essential for resource, and it is used for day-to-day life. Due to increased industrial process, both fresh water and marine water ecosystem contaminated. In industrial effluents, huge variety and classes of both organic and inorganic dyes, bleaching components used and it is directly affects natural water bodies by polluting the water quality. Literature survey showed that, various types of biopolymers which include cellulose, chitosan, chitin, alginate, xanthan gum, dextrin, carrageenan, lignin, protein are as adsorbents to remove dye. In this experimentation, initially, chitin was extracted from fish scale by optimizing demineralization, deproteination, and contact time. To screen optimum condition, Normality of HCL, Molarity of NaOH and contact time (Hours) studied to extract chitin from fish scale. Based on yield, fish scale and chitin were used as natural bio adsorbent to remove methylene blue and acid green from aqueous solution.