Cyber-Physical Systems (CPS) refer to integrated computational and physical processes, where computational elements monitor and control physical processes, usually with a feedback loop. Analysis of trust formation within a complex networks of linked social, physical and computational entities has a potential to reveal otherwise implicit trustworthiness requirements and to improve the CPS design and acceptance. We address this challenge with a method for trust analysis that supports the alignment between trust concerns expressed by the prospective CPS users and stakeholders, trustworthiness requirements formulated by the CPS designers and technical elements of the CPS to be developed. We apply this method to a real CPS design project. Our case study shows that the explicit analysis of trust not only improves traceability between user trust concerns and technical features of the CPS, but also allows for identification of new relevant trustworthiness requirements, affecting the system design and acceptance.