The research developed within this Toolkit stems from an inter-, trans-, and cross-disciplinary collaboration including marine sciences, climate and ocean governance, ocean policy, marine ecology, marine socio-ecology, planetary health, and transdisciplinary science. This chapter explores the definitions and applications of inter-, trans-, and cross-disciplinary approaches within the context of ocean literacy research and education. The primary objective of this exploration is to develop a learning activity for young people that integrates inter-, cross-, and transdisciplinarity into ocean literacy education. The overall aim of this Toolkit chapter is to advance ocean literacy’s role in ocean protection and sustainability efforts through inter-, cross-, and transdisciplinary approaches to research and education.