Fluoride (F), present naturally in soil and water, is a lethal inorganic pollutant that needs to be treated immediately even at lower amounts. It interacts with flora, fauna, and other living things, including bacteria, to disrupt many metabolic pathways and adversely influence a range of physiochemical properties. Because conventional F remediation methods are costly, labor-intensive, and time-consuming, they are not practical for sustainable agriculture. Higher plants, fungus, bacteria, and algae are used as viable substitutes in biotechnological techniques such as bioremediation to control F contamination, rehabilitate polluted soil, as well as improve crop. The efficiency of natural native factors may be improved and preferred above dangerous substances in sustainable agriculture. With an emphasis on their application in cleaning up F-contaminated areas, this chapter examines the potential applications of a number of biological approaches in environmental cleanup. Furthermore, it highlights the importance of a thorough systematic review of modern biotechnological approaches to enhance plant-microbe relations and encourage fluoride degradation. This demonstrates most current developments in the field of treating F-polluted environments with microbial assistance.