Due to the excessive release of toxic and hazardous compounds into the environment, environmental pollution is increasing day by day. This rapid increase in pollutants results from industrialization and urbanization expansion. This pollutant affects in a harmful way to all the living forms, alters the environmental conditions and ecology, and changes diversity manner. The removal of these pollutants has been attempted using numerous conventional approaches, but they have not proven very successful. Until now microbial bioremediation represents the safest way to turn pollutants into non-hazardous materials. Soil ecosystem contains diverse microorganisms with a large capacity of removing a large number of pollutants through their unique enzymatic power. Microbial enzymes are proven to be an efficient and environmentally friendly technology solution for detoxifying pollutants from the environment. Many soil organic pollutants can be remedied by enzymes, including organo-heavy metals, PAHs, azo dyes, and polymers. Hydrolase enzymes (esterase, protease, lipase, cellulase, cutinase), and organophosphorus hydrolase enzymes (oxygenase, peroxidase, laccase) are the main pollutant-remediating soil enzymes. The current state of soil pollution, harmful pollutants’ impacts on soil life forms, as well as the microbial bioremediation enzymes, types, mechanisms, and possible applications, will be discussed in this chapter.