The verification of crew workload airworthiness compliance is one of the core contents of civil aircraft airworthiness certification. This paper takes the workload assessment of pilot approach and landing as the research object, and attempts to establish a pilot approach and landing workload evaluation method for crew workload airworthiness certification. Firstly, interpret the airworthiness clause CCAR25.1523 for crew workload and attempt to establish a compliance verification method that combines subjective evaluation with physiological evaluation. Through experimental design, Tobii eye tracking device and NASA-TLX scale were used to collect physiological indexes of eye movement and subjective survey data of participants. Four arithmetic operations were used for indicator blending and correlation analysis. The homogeneity of variance test analysis showed that the two indexes of gaze time smoothness ratio and average pupil rate of variation were statistically significant. The conclusion indicates that the smoothness ratio of gaze time and the average rate of pupil variation can be used to evaluate the workload of pilots during approach and landing. It shows that as the workload increases, the fluctuation amplitude of the smoothness ratio of gaze time is smaller, and the ratio is also smaller. Conversely, as the workload decreases, the fluctuation amplitude and ratio of the smoothness ratio of gaze time are larger. When the workload is higher, the fluctuation area of the average pupil variation rate coincides with the normal workload area, which is smaller. Conversely, the fluctuation area of the average pupil variation rate coincides with the normal workload area, which is a characteristic of the law.