This investigation extends prior research on the impact of audio stimuli in Virtual Reality (VR) environments, with an enhanced focus on understanding the relationship between audio stimuli and the level of immersion, as indicated by stress levels. Using the State-Trait Anxiety Inventory (STAI) for psychological assessment and heart rate variability for physiological responses, this study examines the interplay of visual and auditory stimuli across four diverse VR scenarios. These scenarios include combinations of city and forest environments paired alternately with matching and contrasting auditory cues. Correlation analysis was employed to determine how audio stimuli influence the level of immersion, reflected through variations in heart rate and STAI-derived stress indicators. Participants’ responses were continuously monitored for real-time physiological effects, while stress assessments were conducted after exposure to each scenario. This methodical approach aims to uncover significant sensory interactions that affect immersion in VR, potentially guiding the design and application of VR technologies across various disciplines.