Addressing the problem that “CO2 flooding EOR has a wide application prospect, but there is no comprehensive evaluation system of its influencing factors”, this study initially develops a comprehensive evaluation framework for assessing the CO2 flooding factors, analyzing the effectiveness and adaptability of relevant evaluation outcomes, and integrating laboratory experiments and numerical simulation research. Subsequently, according to the geological conditions, fluid properties and gas injection development characteristics of Huang 3 block in Jiyuan Oilfield, taking oil recovery as an index, the weight of quantitative influencing factors are determined using the Analytic Hierarchy Process and the main controlling factors are identified and screened. Finally, it is comprehensively evaluated by fuzzy evaluation and the principle of maximum membership degree. The results show that the influencing factors about three aspects, mainly including 14 factors such as oil saturation, heterogeneity, permeability, effective thickness, reservoir temperature, original formation pressure, dip angle, crude oil density, crude oil viscosity, minimum miscible pressure, gas injection rate, injection pressure, bottom hole flowing pressure and well pattern density. The weight of oil saturation is the highest, the heterogeneity is the second, and together with permeability, gas injection rate, injection pressure, bottom hole flowing pressure, crude oil density, crude oil viscosity and minimum miscible pressure constitute the main controlling factors. Moreover, the evaluation results align with actual production outcomes, confirming the method’s accuracy and scientific validity. The findings offer valuable insights for key factors in CO2 flooding technology, optimizing design schemes, and refining EOR strategies.