The genome editing methods have unlocked new possibilities for targeted, significant trait improvement in various crops, such as industrial crops, fruit crops, ornamental crops, and all agricultural crops. However, these genome editing technologies have not been limited to agricultural crops; they have been carried out in various categories of living things, like microorganisms as well as animals, and also in numerous areas such as industries, basic research, and life science. This biotechnologically innovative tool is the favored option for the alterations of genetic material in various categories of living things due to its better level of advanced efficiency and accuracy and overall decrease in off-target gene alterations compared with zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). Although the clustered regularly interspaced short palindromic repeat-based genome editing system has not required any regulatory tests or ethical issues related to these plants being accepted by the public compared with transgenic technology. This genome editing technology is successfully applied to enhance several genetic traits in horticultural and industrial crops, like increasing biotic and abiotic tolerance, transforming plant architecture, and increasing the accumulation of desired metabolites. CRISPR-Cas9 is now being used in many laboratories to mutate specific genomic DNA sequences. This book chapter discusses the potential of the CRISPR-based genome editing process for enhancing commercially valuable traits like higher nutritional value, protein content, and vitamin content, as well as the advancement of useful industrial products like rubber, biofuel, fiber products, and medicines.