Microgreens, young edible greens harvested at the cotyledon or first true leaf stage, have gained popularity due to their high nutritional content, concentrated flavors and vibrant colors. Among microgreens, leguminous varieties offer unique health benefits attributed to their rich protein and phytochemical profiles. This chapter delves into the advancements in cultivation substrates and methods including optimization of growth substrates, encompassing organic materials, hydroponic systems, vertical farming techniques, biofortification and novel blends, tailored to enhance the nutritional quality and production efficiency of leguminous microgreens. Furthermore, the chapter explores the influence of environmental factors including light intensity, photoperiod, and temperature on the growth and phytochemical accumulation of leguminous microgreens. By leveraging controlled environment agriculture (CEA) technologies, such as LED lighting and climate control systems, precise environmental conditions can be maintained to promote optimal growth and nutritional enrichment. The findings suggest that tailored cultivation substrates and innovative production methods can significantly elevate the nutritional quality and yield of leguminous microgreens. These advancements not only offer opportunities for sustainable food production but also contribute to promoting health-conscious dietary choices in modern gastronomy.