In recent years, the development of web applications for teaching and learning has become a focus of research, leading professionals from different fields to form interdisciplinary teams to work together on the integration and development of such tools. Requirements engineering has enabled researchers to identify and prioritize the needs and specifications that these technological tools must meet in order to have the greatest possible impact on the training process. From the implementation of semi-structured interviews, the evaluation of the needs of the environment, and the analysis of the state of the art, the methodological phases for the requirements engineering of web applications developed for the learning of topics related to the field of the health sciences are approached and prioritized. The methodological and strategic aspects applied in the elicitation of five groups of requirements are presented: (i) content, (ii) pedagogical, (iii) functional, (iv) non-functional, and (v) technical. All groups consider the synergy with the actors involved in the process of developing the application to support the learning of students belonging to health sciences or related programmes. The interaction and prioritization of the strategies proposed for the collection of requirements have allowed the generation of a guide for the fluid, clear and organized development of a web application for teaching in the health sciences. In which the involvement and high synergy of the actors involved promote the development of technological tools that have a greater impact on the acquisition and reinforcement of knowledge and skills.