Whole building life cycle assessment (WBLCA) is one of the most important methodologies to reduce the environmental impacts in the building sector. However, research studies usually face challenges in presenting comprehensive life cycle assessment (LCA) results due to the complexity of assessments at the building level. In addition, limited methods are available to systematically evaluate and optimise the whole building LCA performance of buildings at the design stage. Few commercial tools, such as Building Information Modelling (BIM) Revit, Athena LCA, and One Click LCA, start to provide quick and convenient assessments to support designers in understanding the overall building life cycle performance. However, there is limited research that analyses the details of the building level LCA results from different tools. This study aims to conduct a whole-building LCA for a single-family residential building in North America with both Revit Add-in Tally and Athena LCA database. The whole-building LCA results similarities and variances were analysed between two software tools; the results show that Global Warming Potential and Eutrophication Potential results are similar, while the other impact factors’ results differentiate significantly. Future research should be conducted to observe all the commercial software tools, investigate the variance and similarities, and propose application guidelines for the construction industry to improve LCA results accuracy and consistency.