This review study investigates the many synthetic strategies employed for the production of magnetite-based ferrite nanoparticles (MFe2O4), with a specific emphasis on their possible usefulness in combating bacterial infections. The aforementioned techniques encompass co-precipitation, hydrothermal, sol–gel, and solvothermal methodologies, each presenting distinct advantages in terms of particle dimensions, dispersion characteristics, and surface attributes. The study also investigates the doping of MFe2O4 nanoparticles with diverse metals in order to augment their magnetic and antibacterial characteristics. The review finds that the antibacterial efficacy of MFe2O4 nanoparticles is significant, since having the capacity to generate reactive oxygen species and disrupt the integrity of bacterial cell membranes. MFe2O4 nanoparticles have considerable potential as a class of antibacterial agents.