Lime is a key ingredient in masonry, and quicklime is a main raw material of Autoclaved Aerated Concrete (AAC) and Calcium Silicate Units (CSU). In mortars, plasters and renders, hydrated lime not only provides workability and water retention during installation, it also provides ductility and water vapor permeability to the installed masonry system. Through carbonation, lime is sequestrating up to 95% of the process CO2 emitted. In blocks, lime is carbonating by the end of life of the products, recapturing 95% of the process CO2. In renders, lime allows for sequestration of up to 90% of the process CO2 emitted during hardening up to 10mm depth after 3 months, and 20 mm after 1 year. This roadmap presents a reference pathway for the European lime sector to achieve negative CO2 emissions by 2050. By 2050, it is estimated that the European lime sector will deliver negative emissions. This will be achieved through full deployment of decarbonisation technologies and techniques (crucially carbon capture, utilization and storage (CCUS), fuel switch to decarbonised/low carbon energy vectors and energy-efficient/electrified kiln technologies) as well as by carbon removal from the atmosphere through lime carbonation during its use phase. The roadmap further explores the key prerequisites that will allow the industry to deliver a reduction in emissions. Altogether, this research demonstrates and quantifies the potential of the European lime sector to deliver net negative emissions by 2050, thus contributing to the overall carbon neutrality of European society.