While recent low-light image enhancement (LLIE) methods have made significant advancements, they still face challenges in terms of low visual quality and weak generalization ability when applied to complex scenarios. To address these issues, we propose a semi-supervised method based on latent mean-teacher and Gaussian process, named LMT-GP. We first design a latent mean-teacher framework that integrates both labeled and unlabeled data, as well as their latent vectors, into model training. Meanwhile, we use a mean-teacher-assisted Gaussian process learning strategy to establish a connection between the latent and pseudo-latent vectors obtained from the labeled and unlabeled data. To guide the learning process, we utilize an assisted Gaussian process regression (GPR) loss function. Furthermore, we design a pseudo-label adaptation module (PAM) to ensure the reliability of the network learning. To demonstrate our method’s generalization ability and effectiveness, we apply it to multiple LLIE datasets and high-level vision tasks. Experiment results demonstrate that our method achieves high generalization performance and image quality. The code is available at https://github.com/HFUT-CV/LMT-GP .