User-centered design (UCD) methodologies are focused on creating solutions adapted to the specific needs and contexts of targeted users. Such practices are especially critical in healthcare settings, where the quality, accessibility, adaptability and usability of diagnostic and treatment tools significantly impact patient outcomes. A key diagnostic tool in clinical practice is the antibiogram, which presents sensitivity test results for microorganisms against various antibiotic concentrations. Interpreting antibiogram results requires customization based on individual clinical factors, adding complexity to the decision-making process in antibiotic therapy. This scoping review aims to improve healthcare research projects, such as the optimization of antibiogram-directed antibiotic therapy, by taking into account the needs of all stakeholders involved. Our contribution provides a critical and descriptive analysis of the application of various UCD methods within healthcare settings and outlines validated methods used in the collection and analysis of data from healthcare studies employing these methodologies. Additionally, the review offers detailed practical application guidelines to assist other researchers in their implementation.