The primary aim of this research is to establish and enhance the biodiesel production capability of a local semi-industrial-scale manufacturing unit that operates dependently on renewable energy sources, while also valorizing the potential of Used Cooking Oil. To achieve this objective, a biodiesel production unit comprising two stainless steel tanks: the large conical and the small one is established, along with supplementary auxiliary modules essential for its operation, such as a photovoltaic system for power supply, pumps, valves, and piping. The entire unit is mounted on a detachable metal framework. The optimal conditions for biodiesel production, including catalyst concentration (1%), reaction temperature (60 °C), and alcohol/oil molar ratio (of 6:1), were determined from subsequent research conducted in the laboratory. These conditions facilitated the achievement of a biodiesel yield of approximately 96%. The study concludes by evaluating the physicochemical properties of the biodiesel produced under these conditions, the results clearly demonstrate that obtaining satisfactory quality biodiesel complies with international standards and conventional algerien diesel.