Unlocking Potential: Improved Optical Approach for Enhanced Plant Stress and Metabolism Analysis with 640 nm Integration

This study explores the evolution of non-destructive optical methodology to analyze plant stress and metabolism, emphasizing the integration of a 640 nm wavelength. Preliminary applications revealed various plant changes related to light deficiency, water scarcity, nutrient deprivation, and pathogen presence. Inspired by these findings, we upgraded our approach to include the 640 nm wavelength, renowned for its role in photosynthesis processes. This modification aims to provide novel insights into stress detection. The methodology, comprising 20 independent channels with LED diodes at 665 nm and 640 nm, was tested on diverse plant species under varying conditions. The outcomes identify two circadian rhythms, demonstrating the potential to detect stress-induced variations. This innovative approach advances nondestructive plant analysis, offering sensitivity in detecting stress under diverse conditions and promising applications in agriculture. The study marks a significant step forward in environmental monitoring and contributes to future developments in plant science and agricultural practices.